TMB High Sensitive One Component ELISA Substrate-TMHST

QYM/SURFTECH TMB ELISA Substrate series features low background, high stability, minimal lot-to-lot variation, and options for different sensitivity levels. It provides an excellent signal response system suitable for the development and production of ELISA kits.TMB High Sensitive One Component ELISA Substrate has high sensitivity, low background and and reliable stability, which is suitable for detection assays with a chromogenic reaction time of 10–15 minutes.

Item no: TMHST

Package size: 1L, 5L, 10L, custom

pH value: 3.6±0.4

Shelf life: 3 years

Storage: 2-8℃

Application: ELISA

Instruction:

QYM/SURFTECH TMB High Sensitive One Component ELISA Substrate should be equilibrated to room temperature before use.

ELISA Chromogenic Reaction Procedure：

Measurement at 450 nm:

1. Wash: After HRP conjugate incubation, wash the plate 3 times with PBST/TBST washing buffer (containing surfactant).

2. Chromogenic reaction: Add 100 μL substrate and incubate at 20–37 °C for the appropriate time, color is blue;

3. Stop: Add 100 μL TMB Stop Solution 450, the color will change yellow;

4. Read: Measure the absorbance at 450 nm within 30 minutes.

Measurement at 650nm：

1. Wash: After HRP conjugate incubation, wash the plate 3 times with PBST/TBST washing buffer (containing surfactant).

2. Chromogenic reaction: Add 100 μL substrate and incubate at 20–37 °C for the appropriate time, color is blue;

3. Stop: Add 100 μL TMB Stop Solution 650, the color remains blue ;

4. Read: Measure the absorbance at 650 nm within 30 minutes.

Kinetic measurement at 650 nm:

1. Wash: After HRP conjugate incubation, wash the plate 3 times with PBST/TBST washing buffer (containing surfactant).

2. Chromogenic reaction: Add 100 μL substrate and incubate at 20–37 °C.

3. Read: Measure the absorbance at 650 nm at set intervals (e.g., every 1 minute).

For research use only or for further use in the production of detection kits. Not for human or animal use!