BCIP/NBT Blue One Component Membrane Substrate-BCIBT

QYM/SURFTECH series of BCIP/NBT membrane/precipitation substrates feature low background, high stability, sensitivity, and minimal batch-to-batch variation. The series provides blue and purple signal systems, along with BCIP/NBT substrates dedicated for immunohistochemistry (IHC). BCIP/NBT Blue One Component Membrane Substrate produces a dark blue signal and is suitable for applications including Western blot and microarray analysis.

Item no: BCIBT

Package size: 1L, 5L, 10L, Custom

pH value: 9.8+/-0.3

Shelf life: 3 years

Storage: 2-8C

Application:

Western blot, Northern blot, Microarray, ELISpot etc.

Instruction:

QYM/SURFTECH BCIP/NBT Blue One Component Membrane Substrate is ready-to-use and should be equilibrated to the reaction temperature before application.

Chromogenic Reaction Procedure (Western Blot):

1. Wash: After alkaline phosphatase (AP) conjugate incubation, wash the membrane 3 times with TBST washing buffer.

2. Chromogenic Reaction: Add 1 mL substrate and incubate at 20-37 C until bands appear dark blue.

3. Stop: Wash 2-3 times with 1.5 mL deionized water to terminate the reaction before background color develops.

4. Detection: Analyze bands by visual inspection or by imaging to quantify gray-scale values.

For research use only or for further use in the production of detection kits. Not for human or animal use!