TMB Green One Component Membrane Substrate-TMMGT

QYM/SURFTECH series of membrane/precipitation substrates features low background, high stability, sensitivity, and minimal batch-to-batch variation. The series provides blue, purple, and dark green signal systems for flexible selection in kit development and production. TMB Green One Component Membrane Substrate produces a dark green-blue precipitate and is suitable for applications such as Western blot and microarray analysis.

Item no: TMMGT

Package size: 1L, 5L, 10L, Custom

pH value: 4.5+/-0.5

Shelf life: 3 years

Storage: 2-8C

Application:

Western blot, Northern blot, Microarray, ELISpot etc.

Instruction:

QYM/SURFTECH TMB Green One Component Membrane Substrate is ready-to-use and should be equilibrated to the reaction temperature before use.

Chromogenic Reaction Procedure (Western Blot):

1. Wash: After HRP conjugate incubation, wash the membrane 3 times with PBST/TBST washing buffer;

2. Chromogenic Reaction: Add 1 mL substrate and incubate at 20-37 C until bands appear dark blue;

3. Stop: Stop the reaction by washing 2-3 times with 1.5 mL deionized water before background develops;

4. Detection: Analyze bands by visual inspection or by imaging to quantify gray-scale values.

For research use only or for further use in the production of detection kits. Not for human or animal use!