TMB Enhanced One Component Membrane Substrate-TMENT

QYM/SURFTECH series of membrane/precipitation substrates features low background, high stability, sensitivity, and minimal batch-to-batch variation. The series provides blue, purple, and dark green signal systems for flexible selection in kit development and production. TMB Enhanced One Component Membrane Substrate is an enhanced TMB membrane substrate that produces a dark purple precipitate, suitable for applications including Western blot and microarray analysis.

Item no: TMENT

Package size: 1L, 5L, 10L, custom

pH value: 4.5+/-0.5

Shelf life: 3 years

Storage: 2-8C

Application:

Western blot, Northern blot, Microarray, ELISpot etc.

Instruction:

QYM/SURFTECH TMB Enhanced One Component Membrane Substrate is readytouse and should be equilibrated to the reaction temperature before use.

Chromogenic Reaction Procedure (Western Blot):

1. Wash: After HRP conjugate incubation, wash the membrane 3 times with PBST/TBST washing buffer;

2. Chromogenic Reaction: Add 1 mL substrate and incubate at 20-37 C until bands appear dark purple;

3. Stop: Stop the reaction by washing 2-3 times with 1.5 mL deionized water before background develops;

4. Detection: Analyze bands by visual inspection or by imaging to quantify grayscale values.

For research use only or for further use in the production of detection kits. Not for human or animal use!