TMB One Component Membrane Substrate-TMMST

QYM/SURFTECH series of membrane/precipitation substrates features low background, high stability, sensitivity, and minimal batch-to-batch variation. The series provides blue, purple, and dark green signal systems for flexible selection in kit development and production. TMB One Component Membrane Substrate produces a dark blue precipitate and is suitable for applications such as Western blot and microarray analysis.

Item no: TMMST

Package size: 1L, 5L, 10L, Custom

pH value: 4.5+/-0.5

Shelf life: 3 years

Storage: 2-8C

Application:

Western blot, Northern blot, Microarray, ELISpot etc.

Instruction:

QYM/SURFTECH TMB Blue One Component Membrane Substrate is ready-to-use and should be equilibrated to the reaction temperature before use.

Chromogenic Reaction Procedure (Western Blot):

1. Wash: After HRP conjugate incubation, wash the membrane 3 times with PBST/TBST washing buffer;

2. Chromogenic Reaction: Add 1 mL substrate and incubate at 20-37 C until bands appear dark blue;

3. Stop: Stop the reaction by washing 2-3 times with 1.5 mL deionized water before background develops;

4. Detection: Analyze bands by visual inspection or by imaging to quantify grayscale values.

For research use only or for further use in the production of detection kits. Not for human or animal use!